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Mouse anti-REDD1 Monoclonal Antibody (1G11)

Catalogue number:
A500-001A-T
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£105.00 Estimated delivery Fri 8 Jul
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Mouse anti-REDD1 Monoclonal Antibody (1G11) - 20 µl (2 blots)

  • Applications: WB, IP, ICC
Product Type:

Antibodies

Alternative Names:

Dig2, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, protein regulated in development and DNA damage response 1, REDD1, REDD-1

Species Reactivity:

Human

Storage Temp:

2 - 8°C

Antibody Host:

Mouse

Antibody Isotype:

Whole IgG

Antibody Type:

Monoclonal

UNSPSC Code:

12352203

Detection of human REDD1 by western blot

Detection of human REDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL) [50 µg for WB; 1mg for IP, 20% of IP loaded] from HeLa cells. Lysate was prepared from untreated (-) cells or cells treated (+) with CoCl2. Antibodies: Mouse monoclonal anti-REDD1 antibody [1G11] (A500-001A-T lot 1) was used at 1:1000 for WB and 3 µl/mg of lysate for IP. REDD1 was also immunoprecipitated (lanes 5&6) by rabbit anti-REDD1 antibody (A302-169A). Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 10 seconds.

Detection of human REDD1 by immunocytoch

Detection of human REDD1 by immunocytochemistry. Sample: Formaldehyde-fixed, CoCl2 treated, asynchronous HeLa cells. Antibody: Mouse monoclonal anti-REDD1 [1G11] (A500-001A-T lot 2) used at a dilution of 1:250. Secondary: FITC-conjugated goat anti-mouse IgG antibody (A90-116F).

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