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  2. Bethyl Laboratories, Inc.
  3. Mouse anti-REDD1 Monoclonal Antibody [1G11]
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Mouse anti-REDD1 Monoclonal Antibody [1G11]

Catalogue number:
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A500-001A-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
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  • Rest of World
£128.00
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Estimated delivery Fri 3 May
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Applications:

WB, IP, ICC

Antibody Host:

Mouse

Target:

REDD1

Species Reactivity:

Human

Antibody Type:

Monoclonal

Immunogen:

human REDD1

Alternative Names:

protein regulated in development and DNA damage response 1; REDD1; HIF-1 responsive protein RTP801; DNA damage-inducible transcript 4 protein; Dig2; REDD-1

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Product Description:

Mouse anti-REDD1 Monoclonal Antibody [1G11] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human REDD1 by western blot
Detection of human REDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL) [50 µg for WB; 1mg for IP, 20% of IP loaded] from HeLa cells. Lysate was prepared from untreated (-) cells or cells treated (+) with CoCl2. Antibodies: Mouse monoclonal anti-REDD1 antibody [1G11] (A500-001A-T lot 1) was used at 1:1000 for WB and 3 µl/mg of lysate for IP. REDD1 was also immunoprecipitated (lanes 5&6) by rabbit anti-REDD1 antibody (A302-169A). Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human REDD1 by immunocytoch
Detection of human REDD1 by immunocytochemistry. Sample: Formaldehyde-fixed, CoCl2 treated, asynchronous HeLa cells. Antibody: Mouse monoclonal anti-REDD1 [1G11] (A500-001A-T lot 2) used at a dilution of 1:250. Secondary: FITC-conjugated goat anti-mouse IgG antibody (A90-116F).
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