ARSA1, ARSA-I, Arsenical pump-driving ATPase, Arsenite-stimulated ATPase, ASNA-I, ATPase ASNA1, GET3, golgi to ER traffic 3 homolog, hARSA-I, hASNA-I, transmembrane domain recognition complex 40 kDa ATPase subunit, TRC40
Between 1 and 50
2 - 8°C
Detection of human and mouse ASNA1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASNA1 antibody A305-452A (lot A305-452A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human ASNA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASNA1 antibody A305-452A (lot A305-452A-1) used for IP at 6 µg per reaction. ASNA1 was also immunoprecipitated by rabbit anti-ASNA1 antibody BL21638. For blotting immunoprecipitated ASNA1, A305-452A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.