Antibody was affinity purified using an epitope specific to LIMCH1 immobilized on solid support. The epitope recognized by A304-933A-T maps to a region between residue 250 to 300 of human LIM and calponin homology domains-containing protein 1 using the numbering given in entry Q9UPQ0.4 (GeneID 22998).
LIM and calponin homology domains-containing protein 1, LIMCH1A, LMO7B
Between 250 and 300
2 - 8°C
Detection of human LIMCH1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LIMCH1 antibody A304-933A-T (lot A304-933A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.