Antibody was affinity purified using an epitope specific to NF1 immobilized on solid support. The epitope recognized by A304-931A-T maps to a region between residue 1 to 50 of human Neurofibromin 1 using the numbering given in entry NP_001035957.1 (GeneID 4763). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
neurofibromatosis 1, neurofibromatosis-related protein NF-1, neurofibromin, NFNS, truncated neurofibromin 1, VRNF, WSS
Between 1 and 50
2 - 8°C
Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody A304-931A-T (lot A304-931A-T-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies A304-932A and A300-140A. For blotting immunoprecipitated NF1, A304-931A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.