Rabbit anti-HECTD3 Antibody, Affinity Purified

Name: Rabbit anti-HECTD3 Antibody, Affinity Purified
Brand: Bethyl Laboratories, Inc.
SKU: CBSKU_100613056
Price: 122 GBP
Availability: InStock

Catalogue number:

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A304-924A-T

Supplier:

Bethyl Laboratories, Inc.

Size:

10 µl (1000 µg/ml) _$$_

Product is available in:

Currency:
GBP
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£122.00

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Estimated delivery Fri 3 May
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Applications:

WB, IP

Antibody Host:

Rabbit

Target:

HECTD3

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 811 and 861

Alternative Names:

E3 ubiquitin-protein ligase HECTD3; HECT domain containing E3 ubiquitin protein ligase 3; HECT domain-containing protein 3; HECT-type E3 ubiquitin transferase HECTD3

Product Description:

Rabbit anti-HECTD3 Antibody, Affinity Purified - 10 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse HECTD3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HECTD3 antibody A304-924A-T (lot A304-924A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human HECTD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HECTD3 antibody A304-924A-T (lot A304-924A-T-1) used for IP at 6 µg per reaction. HECTD3 was also immunoprecipitated by rabbit anti-HECTD3 antibody BL19755. For blotting immunoprecipitated HECTD3, A304-924A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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