Rabbit anti-Septin 10/SEPT10 Antibody, Affinity Purified - A304-916A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Septin 10/SEPT10 immobilized on solid support.The epitope recognized by A304-916A-T maps to a region between residue 404 to 454 of human Septin-10 using the numbering given in entry Q9P0V9.2 (GeneID 151011).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
IP
Detection of human Septin 10/SEPT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Septin 10/SEPT10 antibody A304-916A-T (lot A304-916A-T-1) used for IP at 6 µg per reaction. Septin 10/SEPT10 was also immunoprecipitated by rabbit anti-Septin 10/SEPT10 antibody A304-915A. For blotting immunoprecipitated Septin 10/SEPT10, A304-916A-T was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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