Antibody was affinity purified using an epitope specific to RABGAP1 immobilized on solid support. The epitope recognized by A304-913A-T maps to a region between residue 25 to 75 of human Rab GTPase-activating protein 1 using the numbering given in entry Q9Y3P9.3 (GeneID 23637). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
GAP and centrosome-associated protein, GAPCENA, rab GTPase-activating protein 1, rab6 GTPase activating protein (GAP and centrosome-associated), Rab6 GTPase activating protein, GAPCenA, rab6 GTPase-activating protein GAPCenA, TBC1 domain family, member 11
Between 25 and 75
2 - 8°C
Detection of human RABGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RABGAP1 antibody A304-913A-T (lot A304-913A-T-1) used for IP at 6 µg per reaction. RABGAP1 was also immunoprecipitated by rabbit anti-RABGAP1 antibody BL19305. For blotting immunoprecipitated RABGAP1, A304-913A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.