Antibody was affinity purified using an epitope specific to DNAJC13 immobilized on solid support. The epitope recognized by A304-872A maps to a region between residue 1175 to 1225 of human DnaJ homolog subfamily C member 13 using the numbering given in entry O75165.5 (GeneID 23317). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DnaJ (Hsp40) homolog, subfamily C, member 13, DnaJ domain-containing protein RME-8, dnaJ homolog subfamily C member 13, PARK21, required for receptor-mediated endocytosis 8, RME8, RME-8
Between 1175 and 1225
2 - 8°C
Detection of human and mouse DNAJC13 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNAJC13 antibody A304-872A (lot A304-872A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human DNAJC13 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNAJC13 antibody A304-872A (lot A304-872A-1) used for IP at 6 µg per reaction. DNAJC13 was also immunoprecipitated by rabbit anti-DNAJC13 antibody BL19117. For blotting immunoprecipitated DNAJC13, A304-872A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.