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Rabbit anti-THOC3 Antibody, Affinity Purified

Catalogue number:
A304-870A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to THOC3 immobilized on solid support. The epitope recognized by A304-870A maps to a region between residue 301 to 351 of human THO complex subunit 3 using the numbering given in entry Q96J01.1 (GeneID 84321). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

hTREX45, TEX1 homolog, THO complex subunit 3, THO3

Species Reactivity:

Human, Mouse

Immunogen:

Between 301 and 351

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse THOC3 by we

Detection of human and mouse THOC3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC3 antibody A304-870A (lot A304-870A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human THOC3 by western blot

Detection of human THOC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC3 antibody A304-870A (lot A304-870A-1) used for IP at 6 µg per reaction. THOC3 was also immunoprecipitated by rabbit anti-THOC3 antibody BL18978. For blotting immunoprecipitated THOC3, A304-870A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.