Antibody was affinity purified using an epitope specific to Formin-like 1/FMNL1 immobilized on solid support. The epitope recognized by A304-869A maps to a region between residue 1025 to 1075 of human Formin-like protein 1 using the numbering given in entry O95466.3 (GeneID 752). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
C17orf1, C17orf1B, CLL-associated antigen KW-13, FHOD4, FMNL, formin-like protein 1, KW-13, leukocyte formin
Between 1025 and 1075
2 - 8°C
Detection of human Formin-like 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Formin-like 1 antibody A304-869A (lot A304-869A-1) used for IP at 6 µg per reaction. Formin-like 1 was also immunoprecipitated by rabbit anti-Formin-like 1 antibody BL18970. For blotting immunoprecipitated Formin-like 1, A304-869A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.