Antibody was affinity purified using an epitope specific to NUDCD3 immobilized on solid support. The epitope recognized by A304-865A-T maps to a region between residue 100 to 150 of human NudC domain-containing protein 3 using the numbering given in entry Q8IVD9.3 (GeneID 23386). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
nudC domain-containing protein 3, NudCL, NudC-like protein
Between 100 and 150
2 - 8°C
Detection of human NUDCD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUDCD3 antibody A304-865A-T (lot A304-865A-T-1) used for IP at 6 µg per reaction. NUDCD3 was also immunoprecipitated by rabbit anti-NUDCD3 antibody A304-866A. For blotting immunoprecipitated NUDCD3, A304-865A-T was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.