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Rabbit anti-Cyclin B2 Antibody, Affinity Purified - A304-509A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Cyclin B2 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-509A-T
Name:
Rabbit anti-Cyclin B2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Cyclin B2 immobilized on solid support.The epitope recognized by A304-509A-T maps to a region between residue 1 to 50 of human Cyclin B2 using the numbering given in entry NP_004692.1 (GeneID 9133).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
G2/mitotic-specific cyclin-B2, HsT17299
Species Reactivity:
Human, Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse Cyclin B2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin B2 antibody A304-509A-T (lot A304-509A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Cyclin B2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin B2 antibody A304-509A-T (lot A304-509A-T-1) used for IP at 6 µg per reaction. Cyclin B2 was also immunoprecipitated by rabbit anti-Cyclin B2 antibody A304-510A. For blotting immunoprecipitated Cyclin B2, A304-510A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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