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Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody, Affinity Purified - A304-408A-T - 20 µl (2 blots) - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody, Affinity Purified
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Size: 20 µl (2 blots)
£89.00
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Catalogue number:
A304-408A-T
Name:
Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to RNA Polymerase II, Phospho (S5) immobilized on solid support.Immunogen for A304-408A-T was a phosphorylated synthetic peptide, which represented a portion of human RNA Polymerase II (GeneID 5430) around serine 5 of the C-terminal repeat YSPTSPS. of human RNA Polymerase II using the numbering given in entry NP_000928.1 (GeneID 5430).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, POLRA, polyme
Species Reactivity:
Human, Mouse
Immunogen:
Surrounding serine 5
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Phospho RNA Polymerase II Poly (S5) by western blot. Samples: Whole cell lysate 50 µ g) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II Poly (S5) antibody A304-408A -T (lot A304-408A- T- 2) was used for WB at 1:1000 (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody A300-653A was used (lower panel). Detection: Chemiluminescence with exposure time of 10 seconds.

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