Antibody was affinity purified using an epitope specific to TFEB immobilized on solid support. The epitope recognized by A303-672A-T maps to a region between residue 325 and 375 of human Transcription Factor EB using the numbering given in entry NP_009093.1 (GeneID 7942).
ALPHATFEB, BHLHE35, class E basic helix-loop-helix protein 35, T-cell transcription factor EB, TCFEB, transcription factor EB
Between 325 and 375
2 - 8°C
Detection of human TFEB by western blot. Samples:Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody A303-672A-T (lot A303-672A-T-3) used for WB at 1:1000. Detection:Chemiluminescence with an exposure time of 10 second.