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Rabbit anti-JHDM1A Antibody, Affinity Purified - A301-476A-T - 2 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-JHDM1A Antibody, Affinity Purified
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Size: 2 µg
£89.00
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Catalogue number:
A301-476A-T
Name:
Rabbit anti-JHDM1A Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to JHDM1A immobilized on solid support.The epitope recognized by A301-476A-T maps to a region between residue 1112 and 1162 of human JmjC domain-containing histone demethylation protein 1A using the numbering given in entry NP_036440.1 (GeneID 22992).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
CXXC8, CXXC-type zinc finger protein 8, FBL11, FBL7, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, F-box/LRR-repeat protein 11, FBXL11, JHDM1A, jmjC domain-containing histone demethylation protein 1A, jumonji C domain
Species Reactivity:
Human, Mouse
Immunogen:
Between 1112 and 1162
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse JHDM1A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JHDM1A antibody A301-476A-T (lot A301-476A-T-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human JHDM1A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JHDM1A antibody A301-476A-T (lot A301-476A-T-2) used for IP at 6 µg per reaction. JHDM1A was also immunoprecipitated by a previous lot of this antibody (lot A301-476A-T-1). For blotting immunoprecipitated JHDM1A, A301-476A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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