Antibody was affinity purified using an epitope specific to CBX8 immobilized on solid support. The epitope recognized by A300-882A-M maps to a region between residue 350 and the C-terminus (residue 389) of human Chromobox homolog 8 using the numbering given in entry NP_065700.1 (GeneID 57332).
chromobox homolog 8 (Pc class homolog, Drosophila), chromobox protein homolog 8, hPc3, Pc class 3 homolog, PC3, polycomb 3, Polycomb 3 homolog, RC1, rectachrome 1
between 350 and C-term
2 - 8°C
Detection of human CBX8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CBX8 antibody A300-882A-M (lot A300-882A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.