Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support. The epitope recognized by A300-439A maps to a region between residue 900 and the C-terminus (residue 940) of human Phosphatase Nuclear Targeting Subunit (a.k.a. Protein Phosphatase 1, regulatory subunit 10) using the numbering given in TrEMBL entry Q96QC0 (GeneID 5514).
CAT53, FB19, HLA-C associated transcript 53, MHC class I region proline-rich protein CAT53, p99, phosphatase 1 nuclear targeting subunit, PNUTS, PP1-binding protein of 114 kDa, PP1R10, Protein FB19, protein phosphatase 1, regulatory (inhibitor) subunit 10
between 900 and C-term
2 - 8°C
Detection of human PNUTS by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody A300-440A-T (lot A300-440A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.