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Rabbit anti-HdmX/MDM4 Antibody, Affinity Purified - A300-287A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-HdmX/MDM4 Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A300-287A-T
Name:
Rabbit anti-HdmX/MDM4 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to HdmX/MDM4 immobilized on solid support.The epitope recognized by A300-287A-T maps to a region between residues 125 and 175 of human ortholog of mouse double minute 4 using the numbering given in entry NP_002384.2 (GeneID 4194).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
Double minute 4 protein, double minute 4, human homolog of, HDMX, mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog, MDM4 protein variant G, MDM4 protein variant Y, MDM4-related protein 1, MDMX, MRP1, p53-binding protein, p53-binding protein
Species Reactivity:
Human
Immunogen:
Between 125 and 175
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate from Jurkat cells (50, 15, 5 µg) prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody A300-287A-T (lot A300-287A-T-3) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody A300-287A-T (lot A300-287A-T-3) used for IP at 6 µg per reaction. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (lot A300-287A-T-2). For blotting immunoprecipitated HdmX/MDM4, A300-287A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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