Antibody was affinity purified using an epitope specific to Bcl-xL immobilized on solid support. The epitope recognized by A300-284A-M maps to a region between residues 1 and 50 of human BCL2-like 1 using the numbering given in SwissProt entry Q07817 (GeneID 598).
apoptosis regulator Bcl-X, BCL2L, Bcl2-L-1, bcl-2-like protein 1, BCLX, Bcl-X, BCL-XL/S, PPP1R52, protein phosphatase 1, regulatory subunit 52
between 1 and 50
2 - 8°C
Detection of human Bcl-xL by western blot. Samples: Whole cell lysate (WCL) from HEK293T cells that were mock transfected or transfected with an expression construct for Bcl-xL. Antibody: Affinity purified rabbit anti-Bcl-xL antibody (Cat. No. A300-284A-M) used for WB at 1:1000. Detection: Chemiluminescence.