*

Rabbit anti-BTAF1 Antibody, Affinity Purified

Catalogue number:
A301-065A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to BTAF1 immobilized on solid support. The epitope recognized by A301-065A maps to a region between residue 1325 and 1375 of human RNA polymerase II, B-TFIID transcription factor-associated, 170kDa using the numbering given in entry NP_003963.1 (GeneID 9044). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Detection of human BTAF1 by western blot
Product Type:

Antibodies

Alternative Names:

ATP-dependent helicase BTAF1, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae), B-TFIID transcription factor-associated 170 kDa subunit, MOT1, Mot1 homolog, TAF(II)170, TAF172, TAF-172, TAFII170, TATA-

Species Reactivity:

Human

Immunogen:

between 1325 and 1375

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human BTAF1 by western blot

Detection of human BTAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody A301-065A used for IP at 3 µg/mg lysate (A and B). BTAF1 was also immunoprecipitated by rabbit anti-BTAF1 antibodies A301-063A and A301-064A, which recognize other epitopes. For blotting immunoprecipitated BTAF1, A301-063A (A) and A301-066A (B) were used at 1 µg/ml,. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 30 seconds (B).