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Rabbit anti-BTAF1 Antibody, Affinity Purified - A301-064A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BTAF1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A301-064A
Name:
Rabbit anti-BTAF1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BTAF1 immobilized on solid support.The epitope recognized by A301-064A maps to a region between residue 1050 and 1100 of human RNA polymerase II, B-TFIID transcription factor-associated, 170kDa using the numbering given in entry NP_003963.1 (GeneID 9044).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

IP

Product Type:
Antibodies
Alternative Names:
ATP-dependent helicase BTAF1, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae), B-TFIID transcription factor-associated 170 kDa subunit, MOT1, Mot1 homolog, TAF(II)170, TAF172, TAF-172, TAFII170, TATA-
Species Reactivity:
Human
Immunogen:
Between 1050 and 1100
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BTAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody A301-064A used for IP at 3 µg/mg lysate (A and B). BTAF1 was also immunoprecipitated by rabbit anti-BTAF1 antibodies A301-063A and A301-065A, which recognize other epitopes. For blotting immunoprecipitated BTAF1, A301-063A (A) and A301-066A (B) were used at 1 µg/ml,. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 30 seconds (B).

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