Antibody was affinity purified using an epitope specific to AKAP8/AKAP95 immobilized on solid support. The epitope recognized by A301-061A maps to a region between residue 575 and 625 of human A-kinase anchor protein 8 using the numbering given in entry NP_005849.1 (GeneID 10270). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
A kinase (PRKA) anchor protein 8, AKAP 95, AKAP-8, AKAP95, AKAP-95, A-kinase anchor protein 8, A-kinase anchor protein 95 kDa, A-kinase anchor protein, 95kDa
between 575 and 625
2 - 8°C
Detection of human AKAP8/AKAP95 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-AKAP8/AKAP95 antibody A301-061A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. AKAP8/AKAP95 was also immunoprecipitated by rabbit anti-AKAP8/AKAP95 antibody A301-062A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).