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Rabbit anti-RBM16 Antibody, Affinity Purified

Catalogue number:
A301-037A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to RBM16 immobilized on solid support. The epitope recognized by A301-037A maps to a region between residue 1221 and the C-terminus (residue 1271) of human RNA binding motif protein 16 using the numbering given in entry NP_055707.3 (GeneID 22828). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

CDC5L complex-associated protein 7, protein SCAF8, RBM16, RNA-binding motif protein 16, SR-like CTD-associated factor 8, SR-related and CTD-associated factor 8

Species Reactivity:

Human, Mouse

Immunogen:

between 1221 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse RBM16 by we

Detection of human and mouse RBM16 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RBM16 antibody A301-037A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. RBM16 was also immunoprecipitated by rabbit anti-RBM16 antibodies A301-036A and BL4483, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

Detection of human RBM16 by immunohistoc

Detection of human RBM16 by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti- RBM16 (Cat. No. A301-037A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB