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Rabbit anti-RBM16 Antibody, Affinity Purified - A301-037A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RBM16 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A301-037A
Name:
Rabbit anti-RBM16 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to RBM16 immobilized on solid support.The epitope recognized by A301-037A maps to a region between residue 1221 and the C-terminus (residue 1271) of human RNA binding motif protein 16 using the numbering given in entry NP_055707.3 (GeneID 22828).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
CDC5L complex-associated protein 7, protein SCAF8, RBM16, RNA-binding motif protein 16, SR-like CTD-associated factor 8, SR-related and CTD-associated factor 8
Species Reactivity:
Human, Mouse
Immunogen:
Between 1221 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse RBM16 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RBM16 antibody A301-037A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. RBM16 was also immunoprecipitated by rabbit anti-RBM16 antibodies A301-036A and BL4483, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

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Detection of human RBM16 by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti- RBM16 (Cat. No. A301-037A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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