Antibody was affinity purified using an epitope specific to NASP immobilized on solid support. The epitope recognized by A301-011A maps to a region between residue 150 and 200 of human nuclear autoantigenic sperm protein (histone-binding) using the numbering given in entry BAD96536.1 (GeneID 4678). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
FLB7527, histone H1-binding protein, HMDRA1, NASP, NASP histone chaperone, nuclear autoantigenic sperm protein, nuclear autoantigenic sperm protein (histone-binding), PRO1999
between 150 and 200
2 - 8°C
Detection of human NASP by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-NASP antibody A301-011A used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.