Antibody was affinity purified using an epitope specific to MOF/MYST1 immobilized on solid support. The epitope recognized by A300-993A maps to a region between residue 417 and the C-terminus (residue 458) of human males absent on the first (MYST histone acetyltransferase 1) using the numbering given in entry BAB13924.1 (GeneID 84148). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
histone acetyltransferase KAT8, histone acetyltransferase MYST1, hMOF, K(lysine) acetyltransferase 8, Lysine acetyltransferase 8, MOF, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1, MYST histone acetyltransferase 1, MYST1, MYST-1, ortholog of Drosophila males
between 417 and C-term
2 - 8°C
Detection of human MOF/MYST1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody A300-993A used for IP at 3 µg/mg lysate. MOF/MYST1 was also immunoprecipitated by rabbit anti-MOF/MYST1 antibodies A300-992A and A300-994A, which recognize upstream epitopes. For blotting immunoprecipitated MOF/MYST1, A300-992A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.