Antibody was affinity purified using an epitope specific to HRS immobilized on solid support. The epitope recognized by A300-988A maps to a region between residue 350 and 400 of human growth factor-regulated tyrosine kinase substrate using the numbering given in entry NP_004703.1 (GeneID 9146). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
hepatocyte growth factor-regulated tyrosine kinase substrate, HRS, human growth factor-regulated tyrosine kinase substrate, protein pp110
between 350 and 400
2 - 8°C
Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HRS antibody A300-988A used for IP at 3 µg/mg lysate. HRS was also immunoprecipitated by rabbit anti-HRS antibody A300-989A, which recognizes a downstream epitope. For blotting immunoprecipitated HRS, A300-989A was used at 1 µg/ml,. Detection: Chemiluminescence with an exposure time of 30 seconds.