Rabbit anti-APC Antibody, Affinity Purified - A300-981A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to APC immobilized on solid support. The epitope recognized by A300-981A maps to a region between residue 2793 and the C-terminus (residue 2843) of human adenomatosis polyposis coli using the numbering given in entry NP_000029.2 (GeneID 324). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
IP, IHC
Detection of human APC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC antibody A300-981A used for IP at 3 µg/mg lysate. APC was also immunoprecipitated by rabbit anti-APC antibodies A300-979A and A300-980A, which recognize upstream epitopes. For blotting immunoprecipitated APC, A300-981A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human APC by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-APC (Cat. No. A300-981A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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