Rabbit anti-APC Antibody, Affinity Purified
Antibody was affinity purified using an epitope specific to APC immobilized on solid support. The epitope recognized by A300-981A maps to a region between residue 2793 and the C-terminus (residue 2843) of human adenomatosis polyposis coli using the numbering given in entry NP_000029.2 (GeneID 324). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Antibodies
adenomatosis polyposis coli tumor suppressor, adenomatous polyposis coli (APC), adenomatous polyposis coli protein, BTPS2, deleted in polyposis 2.5, DP2, DP2.5, DP3, epididymis secretory sperm binding protein, GS, PPP1R46, Protein APC, protein phosphatase
Human
between 2793 and C-term
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human APC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC antibody A300-981A used for IP at 3 µg/mg lysate. APC was also immunoprecipitated by rabbit anti-APC antibodies A300-979A and A300-980A, which recognize upstream epitopes. For blotting immunoprecipitated APC, A300-981A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
