Antibody was affinity purified using an epitope specific to APC immobilized on solid support. The epitope recognized by A300-979A maps to a region between residue 1 and 50 of human adenomatosis polyposis coli using the numbering given in entry NP_000029.2 (GeneID 324). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
adenomatosis polyposis coli tumor suppressor, adenomatous polyposis coli (APC), adenomatous polyposis coli protein, BTPS2, deleted in polyposis 2.5, DP2, DP2.5, DP3, epididymis secretory sperm binding protein, GS, PPP1R46, Protein APC, protein phosphatase
between 1 and 50
2 - 8°C
Detection of human APC by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC antibody A300-979A used for IP at 3 µg/mg lysate. APC was also immunoprecipitated by rabbit anti-APC antibodies A300-980A and A300-981A, which recognize downstream epitopes. For blotting immunoprecipitated APC, A300-981A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.