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Rabbit anti-PPP2R1A Antibody, Affinity Purified

Catalogue number:
A300-963A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to PPP2R1A immobilized on solid support. The epitope recognized by A300-963A maps to a region between residue 540 and the C-terminus (residue 588) of human protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform using the numbering given in entry NP_055040.2 (GeneID 5518). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

medium tumor antigen-associated 61 KDA protein, MRD36, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PP2AA, PP2AAALPHA, PP2A-Aalpha, PR65A, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, protein pho

Species Reactivity:

Human, Mouse

Immunogen:

between 540 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse PPP2R1A by

Detection of human and mouse PPP2R1A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP2R1A antibody A300-963A (lot A300-963A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PPP2R1A by western bl

Detection of human PPP2R1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R1A antibody A300-963A (lot A300-963A-2) used for IP at 3 µg per reaction. PPP2R1A was also immunoprecipitated by rabbit anti-PPP2R1A antibody A300-962A. For blotting immunoprecipitated PPP2R1A, A300-963A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.