Antibody was affinity purified using an epitope specific to THRAP3/TRAP150 immobilized on solid support. The epitope recognized by A300-956A maps to a region between residue 905 and the C-terminus (residue 955) of human Thyroid Hormone Receptor Associated Protein 3 (Thyroid Hormone Receptor-Asscociated Protein, 150 kDa) using the numbering given in entry NP_005110.1 (GeneID 9967). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BCLAF1 and THRAP3 family member 2, BCLAF2, thyroid hormone receptor-associated protein 3, thyroid hormone receptor-associated protein complex 150 kDa component, thyroid hormone receptor-associated protein, 150 kDa subunit, TRAP150, TR-associated protein,
between 905 and C-term
2 - 8°C
Detection of human THRAP3/TRAP150 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-THRAP3/TRAP150 antibody A300-956A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). THRAP3/TRAP150 was also immunoprecipitated by rabbit anti-THRAP3/TRAP150 antibodies A300-955A and BL4215, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of human THRAP3/TRAP150 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- THRAP3/TRAP150 (Cat. No. A300-956A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB