Antibody was affinity purified using an epitope specific to DOT1L immobilized on solid support. The epitope recognized by A300-954A maps to a region between residue1485 and the C-terminus (residue 1537) of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
DOT1, DOT1 like histone H3K79 methyltransferase, DOT1-like histone methyltransferase, DOT1-like protein, DOT1-like, histone H3 methyltransferase, H3-K79-HMTase, histone H3-K79 methyltransferase, histone methyltransferase DOT1L, histone-lysine N-methyltran
between 1485 and C-term
2 - 8°C
Detection of human DOT1L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody A300-954A used for IP at 3 µg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti-DOT1L antibody A300-953A, which recognizes an upstream epitope. For blotting immunoprecipitated DOT1L, A300-953A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time 10 seconds.