Antibody was affinity purified using an epitope specific to KIFC1 immobilized on solid support. The epitope recognized by A300-952A maps to a region between residue 625 and 673 of human Kinesin Family Member C1 using the numbering given in entry NP_002254.1 (GeneID 3833). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HSET, kinesin-like 2, kinesin-like protein 2, kinesin-like protein KIFC1, kinesin-related protein HSET, KNSL2
between 625 and 673
2 - 8°C
Detection of human KIFC1/MKLP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIFC1/MKLP2 antibody A300-952A used for IP at 3 µg/mg lysate. KIFC1/MKLP2 was also immunoprecipitated by rabbit anti-KIFC1/MKLP2 antibodies A300-951A, BL3577, and BL3578, which recognize upstream epitopes. For blotting immunoprecipitated KIFC1/MKLP2, A300-951A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time 30 seconds.