Rabbit anti-TCEB3 Antibody, Affinity Purified

Catalogue number:

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A300-943A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µl _$$_

Product is available in:

N/A

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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Species Reactivity:

Human

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Immunogen:

between 500 and 550

Alternative Names:

EloA, elongin 110 kDa subunit, elongin-A, RNA polymerase II transcription factor SIII subunit A1, SIII, SIII p110, TCEB3, TCEB3A, transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A), transcription elongation factor B alpha subunit,

Data sheet: View or download
MSDS: View or download

Product Description:

Antibody was affinity purified using an epitope specific to TCEB3 immobilized on solid support. The epitope recognized by A300-943A maps to a region between residue 500 and 550 of human Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, elongin A) using the numbering given in entry NP_003189.1 (GeneID 6924). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of human TCEB3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TCEB3 antibody A300-943A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). TCEB3 was also immunoprecipitated by rabbit anti-TCEB3 antibodies A300-942A and BL4209, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human TCEB3 by immunohistochemistry. Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti- TCEB3 (Cat. No. A300-943 Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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