Antibody was affinity purified using an epitope specific to USP36/DUB1 immobilized on solid support. The epitope recognized by A300-940A maps to a region between residue 1070 and the C-terminus (residue 1121) of human Ubiquitin Specific Peptidase 36 (Deubiquinating Enzyme 1) using the numbering given in entry NP_079366.2 (GeneID 57602). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 36, DUB1, ubiquitin carboxyl-terminal hydrolase 36, ubiquitin specific protease 36, ubiquitin thioesterase 36, ubiquitin-specific-processing protease 36
between 1070 and C-term
2 - 8°C
Detection of Human USP36/DUB1 by Western Blot. Samples: Whole cell lysate (50 µg) from HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody A300-940A (lot A300-940A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human USP36/DUB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP36/DUB1 antibody A300-940A (lot A300-940A-2) used for IP at 6 µg per reaction. USP36/DUB1 was also immunoprecipitated by rabbit anti-USP36/DUB1 antibody BL4146. For blotting immunoprecipitated USP36/DUB1, A300-940A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human USP36/DUB1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP36/DUB1(Cat. No. A300-940A Lot 2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB