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Rabbit anti-USP24 Antibody, Affinity Purified - A300-938A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-USP24 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-938A
Name:
Rabbit anti-USP24 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to USP24 immobilized on solid support.The epitope recognized by A300-938A maps to a region between residue 2410 and the C-terminus (residue 2460) of human Ubiquitin Specific Peptidase 24 using the numbering given in entry XP_371254.5 (GeneID 23358).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
deubiquitinating enzyme 24, ubiquitin carboxyl-terminal hydrolase 24, ubiquitin specific protease 24, ubiquitin thioesterase 24, ubiquitin thiolesterase 24, ubiquitin-specific processing protease 24, Ubiquitin-specific-processing protease 24
Species Reactivity:
Human
Immunogen:
Between 2410 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse USP24 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP24 antibody A300-938A (lot A300-938A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human USP24 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP24 antibody A300-938A (lot A300-938A-2) used for IP at 3 µg per reaction. USP24 was also immunoprecipitated by a previous lot of this antibody (lot A300-938A-1) and rabbit anti-USP24 antibody BL4197. For blotting immunoprecipitated USP24, A300-938A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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