Antibody was affinity purified using an epitope specific to PRMT6 immobilized on solid support. The epitope recognized by A300-928A maps to a region between residue 1 and 50 of human Protein Arginine Methyltransferase 6 using the numbering given in entry AAK85733.1 (GeneID 55170). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, histone-arginine N-methyltransferase PRMT6, HMT1 hnRNP methyltransferase-like 6, HRMT1L6, protein arginine N-methyltransferase 6
between 1 and 50
2 - 8°C
Detection of human PRMT6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PRMT6 antibody A300-928A used for IP at 3 µg/mg lysate. For blotting immunoprecipitated PRMT6, rabbit anti-PRMT6 antibody A300-929A was used at 1 µg/ml,. Detection: Chemiluminescence with an exposure time of 30 seconds.