Rabbit anti-PRMT6 Antibody, Affinity Purified - A300-928A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to PRMT6 immobilized on solid support.The epitope recognized by A300-928A maps to a region between residue 1 and 50 of human Protein Arginine Methyltransferase 6 using the numbering given in entry AAK85733.1 (GeneID 55170).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
IP
Detection of human PRMT6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PRMT6 antibody A300-928A used for IP at 3 µg/mg lysate. For blotting immunoprecipitated PRMT6, rabbit anti-PRMT6 antibody A300-929A was used at 1 µg/ml,. Detection: Chemiluminescence with an exposure time of 30 seconds.
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