Antibody was affinity purified using an epitope specific to USP15 immobilized on solid support. The epitope recognized by A300-923A maps to a region between residue 960 and the C-terminus of human Ubiquitin Specific Peptidase 15 using the numbering given in entry AAD41086.1 (GeneID 9958). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 15, ubiquitin carboxyl-terminal hydrolase 15, ubiquitin thioesterase 15, ubiquitin thiolesterase 15, ubiquitin-specific-processing protease 15, UNPH-2, UNPH4
Human, Mouse, Rat
between 960 and C-term
2 - 8°C
Detection of human USP15 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-USP15 antibody A300-923A used for WB at 0.4 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Detection of mouse and rat USP15 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP15 antibody A300-923A (lot A300-923A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.