Antibody was affinity purified using an epitope specific to BCAS2 immobilized on solid support. The epitope recognized by A300-916A maps to a region between residue 870 and the C-terminus (residue 928) of human Breast Carcinoma Amplified Sequence 3 using the numbering given in entry BAB15156.1 (GeneID 54828). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BCAS4/BCAS3 fusion, breast carcinoma amplified sequence 4/3 fusion protein, breast carcinoma-amplified sequence 3, GAOB1, MAAB, metastasis associated antigen of breast cancer, protein Maab1, Rudhira
between 870 and C-term
2 - 8°C
Detection of human BCAS3 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15, and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-BCAS3 antibody A300-916A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BCAS3 was also immunoprecipitated by rabbit anti-BCAS3 antibody BL4013, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 minute (A) and 1 second (B).
Detection of human BCAS3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BCAS3 (Cat. No. A300-916A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB