Rabbit anti-PPP5C Antibody, Affinity Purified

Catalogue number:

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A300-909A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µl _$$_

Product is available in:

N/A

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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Species Reactivity:

Human, Mouse

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Immunogen:

between 1 and 50

Alternative Names:

PP5, PPP5, PPT, PP-T, protein phosphatase T, serine/threonine-protein phosphatase 5

Data sheet: View or download
MSDS: View or download

Product Description:

Antibody was affinity purified using an epitope specific to PPP5C immobilized on solid support. The epitope recognized by A300-909A maps to a region between residue 1 and 50 of human Protein Phosphatase 5, Catalytic Subunit using the numbering given in entry NP_006238.1 (GeneID 5536). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of human and mouse PPP5C by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-PPP5C antibody A300-909A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 5 seconds (B).

Detection of human PPP5C by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PPP5C (Cat. No. A300-909A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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