Antibody was affinity purified using an epitope specific to PPP5C immobilized on solid support. The epitope recognized by A300-909A maps to a region between residue 1 and 50 of human Protein Phosphatase 5, Catalytic Subunit using the numbering given in entry NP_006238.1 (GeneID 5536). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
PP5, PPP5, PPT, PP-T, protein phosphatase T, serine/threonine-protein phosphatase 5
between 1 and 50
2 - 8°C
Detection of human and mouse PPP5C by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-PPP5C antibody A300-909A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 5 seconds (B).