Antibody was affinity purified using an epitope specific to USP10 immobilized on solid support. The epitope recognized by A300-901A maps to a region between residue 300 and 3500 of human Ubiquitin Specific Peptidase 10 using the numbering given in entry NP_005144.1 (GeneID 9100). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 10, ubiquitin carboxyl-terminal hydrolase 10, ubiquitin specific protease 10, ubiquitin thioesterase 10, ubiquitin thiolesterase 10, ubiquitin-specific-processing protease 10, UBPO
between 300 and 3500
2 - 8°C
Detection of human USP10 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-USP10 antibody A300-901A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). USP10 was also immunoprecipitated by rabbit anti-USP10 antibodies A300-900A and BL3979, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).