Antibody was affinity purified using an epitope specific to KIF20A/RAB6KIFL immobilized on solid support. The epitope recognized by A300-879A maps to a region between residue 840 and the C-terminus (residue 890) of human Kinesin Family Member 20A (RAB6 Interacting, Kinesin-Like) using the numbering given in entry NP_005724.1 (GeneID 10112). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
GG10_2, kinesin-like protein KIF20A, mitotic kinesin-like protein 2, MKLP2, RAB6 interacting, kinesin-like (rabkinesin6), rab6-interacting kinesin-like protein, RAB6KIFL, rabkinesin-6
between 840 and C-term
2 - 8°C
Detection of human KIF20A/RAB6KIFL by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-KIF20A/RAB6KIFL antibody BL3708 (Cat. No. A300-879A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). KIF20A/RAB6KIFL was also immunoprecipitated by rabbit anti-KIF20A/RAB6KIFL antibody BL3705 (Cat. No. A300-878A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).