Antibody was affinity purified using an epitope specific to PELP1/MNAR immobilized on solid support. The epitope recognized by A300-876A maps to a region between residue 25 and 75 of human Proline-, Glutamic Acid-, Leucine-Rich Protein 1 (Modulator of Nongenomic Activity of Estrogen Receptor) using the numbering given in entry AAN41255.1 (GeneID 27043). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MNAR, modulator of non-genomic activity of estrogen receptor, P160, proline and glutamic acid rich nuclear protein, proline-, glutamic acid- and leucine-rich protein 1, transcription factor HMX3
between 25 and 75
2 - 8°C
Detection of human PELP1/MNAR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody BL749 (Cat. No. A300-876A) used for IP at 3 µg/mg lysate. PELP1/MNAR was also immunoprecipitated by rabbit anti-PELP1/MNAR antibodies BL750 and BL751 (Cat. No. A300-180A), which recognize downstream epitopes. For blotting immunoprecipitated PELP1/MNAR, BL751 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human PELP1/MNAR by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- PELP1/MNAR (Cat. No. A300-876A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB