Antibody was affinity purified using an epitope specific to CDK5RAP3 immobilized on solid support. The epitope recognized by A300-871A maps to a region between residue 450 and the C-terminus (residue 506) of human CDK5 Regulatory Subunit Associated Protein 3 using the numbering given in entry NP_788276.1 (GeneID 80279). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C53, CDK5 activator-binding protein C53, CDK5 regulatory subunit associated protein IC53-2, CDK5 regulatory subunit-associated protein 3, HSF-27, IC53, ischemic heart CDK5 activator-binding protein C53, LXXLL/leucine-zipper-containing ARFbinding protein,
between 450 and C-term
2 - 8°C
Detection of human CDK5RAP3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CDK5RAP3 antibody BL3564 (Cat. No. A300-871A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). CDK5RAP3 was also immunoprecipitated by rabbit anti-CDK5RAP3 antibody BL3563 (Cat. No. A300-870A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).