Antibody was affinity purified using an epitope specific to PRMT5 immobilized on solid support. The epitope recognized by A300-850A maps to a region between residue 580 and the C-terminus (residue 637) of Protein Arginine Methyltransferase 5 using the numbering given in entry NP_006100.2 (GeneID 10419). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
72 kDa ICln-binding protein, histone-arginine N-methyltransferase PRMT5, HMT1 hnRNP methyltransferase-like 5, HRMT1L5, IBP72, jak-binding protein 1, JBP1, protein arginine N-methyltransferase 5, shk1 kinase-binding protein 1 homolog, SKB1, SKB1 homolog, S
between 580 and C-term
2 - 8°C
Detection of human and mouse PRMT5 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PRMT5 antibody BL3691 (Cat. No. A300-850A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PRMT5 was also immunoprecipitated by rabbit anti-PRMT5 antibodies BL3688 (Cat. No. A300-849A) and BL3690, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).