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Rabbit anti-LEDGF/p75 Antibody, Affinity Purified - A300-848A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-LEDGF/p75 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-848A
Name:
Rabbit anti-LEDGF/p75 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to LEDGF/p75 immobilized on solid support.The epitope recognized by A300-848A maps to a region between residue 480 and the C-terminus (residue 530) of human Lens Epithelium Derived Growth Factor/Transcriptional Coactivator p75 using the numbering given in entry NP_150091.2 (GeneID 11168).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
CLL-associated antigen KW-7, dense fine speckles 70 kDa protein, DFS 70, DFS70, LEDGF, lens epithelium-derived growth factor, p52, p75, PAIP, PC4 and SFRS1-interacting protein, PSIP2, transcriptional coactivator p52/p75, Transcriptional coactivator p75/p5
Species Reactivity:
Human
Immunogen:
Between 480 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human LEDGF/p75 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-LEDGF/p75 antibody BL3656 (Cat. No. A300-848A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). LEDGF/p75 was also immunoprecipitated by rabbit anti-LEDGF/p75 antibody BL3655 (Cat. No. A300-847A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

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