Antibody was affinity purified using an epitope specific to PPP6C immobilized on solid support. The epitope recognized by A300-844A maps to a region between residue 250 and the C-terminus (residue 305) of human Protein Phosphatase 6, Catalytic Subunit using the numbering given in entry NP_002712.1 (GeneID 5537). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
PP6, PP6C, serine/threonine protein phosphatase catalytic subunit, serine/threonine-protein phosphatase 6 catalytic subunit
between 250 and C-term
2 - 8°C
Detection of human and mouse PPP6C by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PPP6C antibody BL3571 (Cat. No. A300-844A) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).