Antibody was affinity purified using an epitope specific to DTX3L/BBAP immobilized on solid support. The epitope recognized by A300-833A maps to a region between residue 75 and 125 of human Deltex 3-Like Protein (B-Lymphoma- and BAL-Associated Protein) using the numbering given in entry NP_612144.1 (GeneID 151636). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BBAP, B-lymphoma- and BAL-associated protein, deltex 3 like, E3 ubiquitin ligase, deltex 3-like, E3 ubiquitin-protein ligase DTX3L, Protein deltex-3-like, rhysin2, rhysin-2, RING-type E3 ubiquitin transferase DTX3L, RNF143
between 75 and 125
2 - 8°C
Detection of human DTX3L/BBAP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DTX3L/BBAP antibody BL3622 (Cat. No. A300-833A) used for IP at 3 µg/mg lysate. DTX3L/BBAP was also immunoprecipitated by rabbit anti-DTX3L/BBAP antibodies BL3623 and BL3624 (Cat. No. A300-834A), which recognize downstream epitopes. For blotting immunoprecipitated DTX3L/BBAP, BL3624 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.