Antibody was affinity purified using an epitope specific to USP4 immobilized on solid support. The epitope recognized by A300-829A maps to a region between residue 225 and 275 of human Ubiquitin Specific Protease 4 using the numbering given in entry AAB72237.1 (GeneID 7375). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 4, ubiquitin carboxyl-terminal esterase 4, ubiquitin carboxyl-terminal hydrolase 4, ubiquitin specific peptidase 4 (proto-oncogene), ubiquitin specific protease 4 (proto-oncogene), ubiquitin thioesterase 4, ubiquitin thiolesterase
between 225 and 275
2 - 8°C
Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP4 antibody BL2194 (Cat. No. A300-829A) used for IP at 3 µg/mg lysate. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody BL2196 (Cat. No. A300-830A), which recognizes a downstream epitope. For blotting immunoprecipitated USP4, BL2196 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.