Antibody was affinity purified using an epitope specific to PIMT immobilized on solid support. The epitope recognized by A300-814A maps to a region between residue 150 and 200 of human PRIP-Interacting Protein with Methyltransferase Domain (Nuclear Receptor Coactivator 6 Interacting Protein) using the numbering given in entry NP_079107.5 (GeneID 96764). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cap-specific guanine-N2 methyltransferase, CLL-associated antigen KW-2, hepatocellular carcinoma-associated antigen 137, NCOA6IP, nuclear receptor coactivator 6-interacting protein, PIMT, PIPMT, PRIP-interacting protein with methyltransferase motif, trime
between 150 and 200
2 - 8°C
Detection of human PIMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PIMT antibody A300-814A (lot A300-814A-2) used for IP at 6 µg per reaction. PIMT was also immunoprecipitated by a previous lot of this antibody (lot A300-814A-1) and rabbit anti-PIMT antibody A304-922A. For blotting immunoprecipitated PIMT, A300-814A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PIMT by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT (Cat. No. A300-814A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB