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Rabbit anti-BAF57/SMARCE1 Antibody, Affinity Purified - A300-810A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BAF57/SMARCE1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-810A
Name:
Rabbit anti-BAF57/SMARCE1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BAF57/SMARCE1 immobilized on solid support.The epitope recognized by A300-810A maps to a region between residue 360 and the C-terminus (residue 411) of human BRG1-Associated Factor 57 (SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin E1) using the numbering given in entry NP_003070.3 (GeneID 6605).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
BAF57, BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, CSS5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
Species Reactivity:
Human, Mouse
Immunogen:
Between 360 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse BAF57/SMARCE1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-BAF57/SMARCE1 antibody A300-810A (lot A300-810A-2) used for WB at 0.1 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BAF57/SMARCE1 was also immunoprecipitated by a previous lot (lot A300-810A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

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Detection of human BAF57/SMARCE1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- BAF57/SMARCE1 (Cat. No. A300-810A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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