Antibody was affinity purified using an epitope specific to BAF57/SMARCE1 immobilized on solid support. The epitope recognized by A300-810A maps to a region between residue 360 and the C-terminus (residue 411) of human BRG1-Associated Factor 57 (SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin E1) using the numbering given in entry NP_003070.3 (GeneID 6605). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BAF57, BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, CSS5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
between 360 and C-term
2 - 8°C
Detection of human and mouse BAF57/SMARCE1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-BAF57/SMARCE1 antibody A300-810A (lot A300-810A-2) used for WB at 0.1 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BAF57/SMARCE1 was also immunoprecipitated by a previous lot (lot A300-810A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human BAF57/SMARCE1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- BAF57/SMARCE1 (Cat. No. A300-810A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB